Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN
To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was uti...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2005-06, Vol.25 (3), p.326-328 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 王家菁 胡志全 叶章群 |
| description | To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN a 2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P |
| doi_str_mv | 10.1007/BF02828157 |
| format | Article |
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The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN a 2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P<0. 05). It was concluded that Trastuzumab with IFN α-2b could inhibit the proliferation of RCC and the expression of HER2 and MRP1 of ACHN and to some extent, reverse the MDR of the tumor cells.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/BF02828157</identifier><language>eng</language><publisher>Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</publisher><subject>HER2 ; MRP1 ; α-2b ; 曲司佐单抗 ; 联合用药 ; 肿瘤细胞</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2005-06, Vol.25 (3), p.326-328</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c981-2a4c729cd160d5d822255560b2805654403829a03a9beab62ce101a1b8ce66723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>王家菁 胡志全 叶章群</creatorcontrib><title>Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN a 2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P<0. 05). It was concluded that Trastuzumab with IFN α-2b could inhibit the proliferation of RCC and the expression of HER2 and MRP1 of ACHN and to some extent, reverse the MDR of the tumor cells.</description><subject>HER2</subject><subject>MRP1</subject><subject>α-2b</subject><subject>曲司佐单抗</subject><subject>联合用药</subject><subject>肿瘤细胞</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpFkE1OwzAQhS0EEqWw4QQWS1BgPI6dZFmillYqBVXdW3bitOmPA0mqttyKi3AmjFqJ1YxG33uj9wi5ZfDIAKKn5wFgjDET0RnpsCThAeMCz_0uIwwg4vySXDXNEkBEEsMOGfeLwmYtrQo6q3XTbr-2G21o6WhabUzpdFtWju7KdkFHgwn9-Q7QUH8Z9qdItcvp6_Sd_al76XByTS4KvW7szWl2yWzQn6XDYPz2Mkp74yBLYhagDrMIkyxnEnKRx4gohJBgMAYhRRgCjzHRwHVirDYSM8uAaWbizEofg3fJw9F2p12h3Vwtq23t_EPVLg-rfL83yqJPCBw48_T9kc7qqmlqW6iPutzo-qAYqL_S1H9pHr47wYvKzT9Lb250tirKtVVMIsNQAv8FvSxl9Q</recordid><startdate>200506</startdate><enddate>200506</enddate><creator>王家菁 胡志全 叶章群</creator><general>Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>200506</creationdate><title>Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN</title><author>王家菁 胡志全 叶章群</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c981-2a4c729cd160d5d822255560b2805654403829a03a9beab62ce101a1b8ce66723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>HER2</topic><topic>MRP1</topic><topic>α-2b</topic><topic>曲司佐单抗</topic><topic>联合用药</topic><topic>肿瘤细胞</topic><toplevel>online_resources</toplevel><creatorcontrib>王家菁 胡志全 叶章群</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>王家菁 胡志全 叶章群</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2005-06</date><risdate>2005</risdate><volume>25</volume><issue>3</issue><spage>326</spage><epage>328</epage><pages>326-328</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN a 2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P<0. 05). It was concluded that Trastuzumab with IFN α-2b could inhibit the proliferation of RCC and the expression of HER2 and MRP1 of ACHN and to some extent, reverse the MDR of the tumor cells.</abstract><pub>Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</pub><doi>10.1007/BF02828157</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2005-06, Vol.25 (3), p.326-328 |
| issn | 1672-0733 1993-1352 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | HER2 MRP1 α-2b 曲司佐单抗 联合用药 肿瘤细胞 |
| title | Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN |
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