Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN

Effect of Trastuzumab in Combination with IFN α-2b on HER2 and MRP1 of ACHN

To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was uti...

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Veröffentlicht in:Journal of Huazhong University of Science and Technology. Medical sciences 2005-06, Vol.25 (3), p.326-328
1. Verfasser: 王家菁 胡志全 叶章群
Format: Artikel
Sprache:eng
Schlagworte:
HER2
MRP1
α-2b
曲司佐单抗
联合用药
肿瘤细胞
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Zusammenfassung:To study the effect of Trastuzumab in combination with IFN α-2b on HER2 and MRP1 of ACHN in vitro, ACHN cell line of RCC was cultured by employing cell culture. The tetrazoliurn-based colorimetric assay was used to evaluate the growth-inhibiting effect of Trastuzumab with IFN α-2b， SP method was utilized to determine the expression of HER2 and MRP1 of the cells. Our results showed that Trastuzumab had inhibitory effect on the growth of renal tumor cells and reversing effect on the multi drug-resistance (MDR) in RCC in a time- and dose-dependent manner. Treated with Trastuzumab with or without IFN a 2b, the expression of HER2 and MRP1 genes of RCC was decreased significantly (P
ISSN:1672-0733
1993-1352
DOI:10.1007/BF02828157