Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87
The expression of CH50 polypoptide in bladder cancer cell line BIU 87 and the effects on the invasion ability of BIU 87 were investigated. The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide wa...
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| Veröffentlicht in: | Current medical science 2005, Vol.25 (3), p.320-322 |
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| creator | 伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化 |
| description | The expression of CH50 polypoptide in bladder cancer cell line BIU 87 and the effects on the invasion ability of BIU 87 were investigated. The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide was detected by using immunohistoehemieal S P method. The expression of the transfeeted gene was identified by RT PCR. Cell invasion assay kit was applied to detect the effect of CH50 polypeptide on the invasion ability of BIU-87. The results showed that the BIU-87 cells transfeeted with pCH510 could express the CH50 polypeptide, while in the control group, no CH50 polypeptide was detectable. In the transfection group, the invasion ability of BIU-87 in vitro was lower than in control group (P |
| doi_str_mv | 10.1007/bf02828155 |
| format | Article |
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The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide was detected by using immunohistoehemieal S P method. The expression of the transfeeted gene was identified by RT PCR. Cell invasion assay kit was applied to detect the effect of CH50 polypeptide on the invasion ability of BIU-87. The results showed that the BIU-87 cells transfeeted with pCH510 could express the CH50 polypeptide, while in the control group, no CH50 polypeptide was detectable. In the transfection group, the invasion ability of BIU-87 in vitro was lower than in control group (P<0.05). It was concluded that CH50 polypeptide was successfully expressed in BIU 87 cells by gene transfeetion, by which the in vitro invasion ability of BIU-87 was inhibited。</description><identifier>ISSN: 1672-0733</identifier><identifier>ISSN: 2096-5230</identifier><identifier>EISSN: 1993-1352</identifier><identifier>EISSN: 2523-899X</identifier><identifier>DOI: 10.1007/bf02828155</identifier><identifier>PMID: 16201284</identifier><language>eng</language><publisher>China: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Pathophysiology, School of Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Biochemistry and Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</publisher><subject>BIU-87 ; Cell Line, Transformed ; CH50 ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Eukaryotic Cells - metabolism ; Fibronectins - biosynthesis ; Fibronectins - genetics ; Genetic Vectors ; Humans ; Neoplasm Invasiveness ; Plasmids - genetics ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Transfection ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; 基因多肽 ; 基因转化 ; 肿瘤入侵 ; 膀胱肿瘤</subject><ispartof>Current medical science, 2005, Vol.25 (3), p.320-322</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2229-cee7aee2e3259940fa081ec4a536e24c1fb0781d25371f04aaa0891b223ff70e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16201284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化</creatorcontrib><title>Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87</title><title>Current medical science</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>The expression of CH50 polypoptide in bladder cancer cell line BIU 87 and the effects on the invasion ability of BIU 87 were investigated. The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide was detected by using immunohistoehemieal S P method. The expression of the transfeeted gene was identified by RT PCR. Cell invasion assay kit was applied to detect the effect of CH50 polypeptide on the invasion ability of BIU-87. The results showed that the BIU-87 cells transfeeted with pCH510 could express the CH50 polypeptide, while in the control group, no CH50 polypeptide was detectable. In the transfection group, the invasion ability of BIU-87 in vitro was lower than in control group (P<0.05). It was concluded that CH50 polypeptide was successfully expressed in BIU 87 cells by gene transfeetion, by which the in vitro invasion ability of BIU-87 was inhibited。</description><subject>BIU-87</subject><subject>Cell Line, Transformed</subject><subject>CH50</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Eukaryotic Cells - metabolism</subject><subject>Fibronectins - biosynthesis</subject><subject>Fibronectins - genetics</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Neoplasm Invasiveness</subject><subject>Plasmids - genetics</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Transfection</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>基因多肽</subject><subject>基因转化</subject><subject>肿瘤入侵</subject><subject>膀胱肿瘤</subject><issn>1672-0733</issn><issn>2096-5230</issn><issn>1993-1352</issn><issn>2523-899X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkTtv2zAQgImgRZImWfoDCqJDhxZqj0dRlMbYyMOAgXZIZoKSjjEdmXJEOYn_fSnYRac78D58vAdjnwX8FAD6V-0ASyyFUifsXFSVzIRU-CHlhcYMtJRn7FOMawClC8xP2ZkoEASW-TnrbpyjZoy8d_yOAvGHwYbpxfeBv_lxxef3CvifvttvaTv6lngqjCvii_Bq40Rd177z434yzDrbtjTwuQ3NFKjr-NIn62zxmJX6kn10tot0dYwX7PH25mF-ny1_3y3m18usQcQqa4i0JUKSqKoqB2ehFNTkVsmCMG-Eq0GXokUltXCQW5uAStSI0jkNJC_Yj4P3Lc1iw5NZ97shpB_NuN4_t-_vtSFM2wAJWCX624HeDv3LjuJoNj42qXUbqN9FU5SFAqgggd8PYDP0MQ7kzHbwGzvsjQAzXcLMbv9dIsFfjtZdvaH2P3pcfQK-Hm2rPjy9-NRmbZtn5zuaIIG5KuVfklOMEg</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化</creator><general>Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Pathophysiology, School of Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Biochemistry and Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87</title><author>伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2229-cee7aee2e3259940fa081ec4a536e24c1fb0781d25371f04aaa0891b223ff70e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>BIU-87</topic><topic>Cell Line, Transformed</topic><topic>CH50</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Eukaryotic Cells - metabolism</topic><topic>Fibronectins - biosynthesis</topic><topic>Fibronectins - genetics</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Neoplasm Invasiveness</topic><topic>Plasmids - genetics</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - genetics</topic><topic>Transfection</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><topic>基因多肽</topic><topic>基因转化</topic><topic>肿瘤入侵</topic><topic>膀胱肿瘤</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Current medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>伍庄 陈忠 叶章群 张健华 叶仕桥 张桂梅 冯作化</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87</atitle><jtitle>Current medical science</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2005</date><risdate>2005</risdate><volume>25</volume><issue>3</issue><spage>320</spage><epage>322</epage><pages>320-322</pages><issn>1672-0733</issn><issn>2096-5230</issn><eissn>1993-1352</eissn><eissn>2523-899X</eissn><abstract>The expression of CH50 polypoptide in bladder cancer cell line BIU 87 and the effects on the invasion ability of BIU 87 were investigated. The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide was detected by using immunohistoehemieal S P method. The expression of the transfeeted gene was identified by RT PCR. Cell invasion assay kit was applied to detect the effect of CH50 polypeptide on the invasion ability of BIU-87. The results showed that the BIU-87 cells transfeeted with pCH510 could express the CH50 polypeptide, while in the control group, no CH50 polypeptide was detectable. In the transfection group, the invasion ability of BIU-87 in vitro was lower than in control group (P<0.05). It was concluded that CH50 polypeptide was successfully expressed in BIU 87 cells by gene transfeetion, by which the in vitro invasion ability of BIU-87 was inhibited。</abstract><cop>China</cop><pub>Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Pathophysiology, School of Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Biochemistry and Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</pub><pmid>16201284</pmid><doi>10.1007/bf02828155</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Current medical science, 2005, Vol.25 (3), p.320-322 |
| issn | 1672-0733 2096-5230 1993-1352 2523-899X |
| language | eng |
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| source | MEDLINE; SpringerNature Journals; Alma/SFX Local Collection |
| subjects | BIU-87 Cell Line, Transformed CH50 Escherichia coli - genetics Escherichia coli - metabolism Eukaryotic Cells - metabolism Fibronectins - biosynthesis Fibronectins - genetics Genetic Vectors Humans Neoplasm Invasiveness Plasmids - genetics Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Transfection Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology 基因多肽 基因转化 肿瘤入侵 膀胱肿瘤 |
| title | Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87 |
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