Effects of Gene Tranfection with CH50 Polypeptide on the Invasion Ability of Bladder Cancer Cell Line BIU-87

The expression of CH50 polypoptide in bladder cancer cell line BIU 87 and the effects on the invasion ability of BIU 87 were investigated. The cukaryotie expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfeetion in vitro. The expression of CH50 polypeptide was detected by using immunohistoehemieal S P method. The expression of the transfeeted gene was identified by RT PCR. Cell invasion assay kit was applied to detect the effect of CH50 polypeptide on the invasion ability of BIU-87. The results showed that the BIU-87 cells transfeeted with pCH510 could express the CH50 polypeptide, while in the control group, no CH50 polypeptide was detectable. In the transfection group, the invasion ability of BIU-87 in vitro was lower than in control group (P