Expression of transforming growth factor-beta in cultured normal human lens epithelia cells
In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2004, Vol.24 (3), p.289-291 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | Huang, Yukan Wei, Houren |
| description | In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action. |
| doi_str_mv | 10.1007/BF02832016 |
| format | Article |
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The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/BF02832016</identifier><identifier>PMID: 15315352</identifier><language>eng</language><publisher>China: Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China</publisher><subject>Cells, Cultured ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Humans ; Lens, Crystalline - cytology ; Lens, Crystalline - metabolism ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Transforming Growth Factor beta - biosynthesis ; Transforming Growth Factor beta - genetics</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2004, Vol.24 (3), p.289-291</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1258-73ad6c3a3cca9a675b7ac0972cf8d009f14bdd1a192a994d48515f5036bde3b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/tjykdxxb-e/tjykdxxb-e.jpg</thumbnail><link.rule.ids>315,781,785,4025,27927,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15315352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yukan</creatorcontrib><creatorcontrib>Wei, Houren</creatorcontrib><title>Expression of transforming growth factor-beta in cultured normal human lens epithelia cells</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><description>In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.</description><subject>Cells, Cultured</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Humans</subject><subject>Lens, Crystalline - cytology</subject><subject>Lens, Crystalline - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Transforming Growth Factor beta - biosynthesis</subject><subject>Transforming Growth Factor beta - genetics</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE2LFDEQhoMo7jp68QdITh6U1krSSTpHXXZVWPCiJw-hOh8zPXYnY9LNzv57e5iBhYK3Dg9vFQ8hbxl8YgD689c74J3gwNQzcs2MEQ0Tkj9fd6V5A1qIK_Kq1j2A1Iq3L8kVk2Idya_Jn9vjoYRah5xojnQumGrMZRrSlm5Lfph3NKKbc2n6MCMdEnXLOC8leJpWDEe6WyZMdAyp0nAY5l0YB6QujGN9TV5EHGt4c8kN-X13--vme3P_89uPmy_3jWNcdo0W6JUTKJxDg0rLXqMDo7mLnQcwkbW99wyZ4WhM69tOMhklCNX7IPpObMjHc-8Dpohpa_d5KWm9aOf9419_PPY2cIAWxMnThrw_04eS_y2hznYa6ulfTCEv1SqlTas4rOCHM-hKrrWEaA9lmLA8Wgb2JN4-iV_hd5fWpZ-Cf0IvpsV_Wy9-Ig</recordid><startdate>2004</startdate><enddate>2004</enddate><creator>Huang, Yukan</creator><creator>Wei, Houren</creator><general>Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2004</creationdate><title>Expression of transforming growth factor-beta in cultured normal human lens epithelia cells</title><author>Huang, Yukan ; Wei, Houren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1258-73ad6c3a3cca9a675b7ac0972cf8d009f14bdd1a192a994d48515f5036bde3b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Cells, Cultured</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - metabolism</topic><topic>Humans</topic><topic>Lens, Crystalline - cytology</topic><topic>Lens, Crystalline - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Transforming Growth Factor beta - biosynthesis</topic><topic>Transforming Growth Factor beta - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yukan</creatorcontrib><creatorcontrib>Wei, Houren</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yukan</au><au>Wei, Houren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of transforming growth factor-beta in cultured normal human lens epithelia cells</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><date>2004</date><risdate>2004</risdate><volume>24</volume><issue>3</issue><spage>289</spage><epage>291</epage><pages>289-291</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.</abstract><cop>China</cop><pub>Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China</pub><pmid>15315352</pmid><doi>10.1007/BF02832016</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2004, Vol.24 (3), p.289-291 |
| issn | 1672-0733 1993-1352 |
| language | eng |
| recordid | cdi_wanfang_journals_tjykdxxb_e200403028 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Cells, Cultured Epithelial Cells - cytology Epithelial Cells - metabolism Humans Lens, Crystalline - cytology Lens, Crystalline - metabolism RNA, Messenger - biosynthesis RNA, Messenger - genetics Transforming Growth Factor beta - biosynthesis Transforming Growth Factor beta - genetics |
| title | Expression of transforming growth factor-beta in cultured normal human lens epithelia cells |
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