Expression of transforming growth factor-beta in cultured normal human lens epithelia cells

In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human...

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Veröffentlicht in:	Journal of Huazhong University of Science and Technology. Medical sciences 2004, Vol.24 (3), p.289-291
Hauptverfasser:	Huang, Yukan, Wei, Houren
Format:	Artikel
Sprache:	eng
Schlagworte:	Cells, Cultured Epithelial Cells - cytology Epithelial Cells - metabolism Humans Lens, Crystalline - cytology Lens, Crystalline - metabolism RNA, Messenger - biosynthesis RNA, Messenger - genetics Transforming Growth Factor beta - biosynthesis Transforming Growth Factor beta - genetics
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Zusammenfassung:	In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.
ISSN:	1672-0733 1993-1352
DOI:	10.1007/BF02832016