Induction of the Expression of Heme Oxygenase Gene in PC12 Cells by Hypoxia

To investigate the expression of the HO-1 gene in PC12 cells in hypoxic environment and gain further insight to the role of HO-1 in cerebral ischemia,PC12 cells were exposed to hyposia environment (95% N2,5% CO2) for 0.5h,1h,4h,8h,12h,24h respectively.The level of HO-1 mRNA was examined by reverse transcriptase polymerase chain reaction (RTPCR);the volume of COHbin the media were measured spectrophotometrically and the cGMP kconcentration of PC12 cell extracts was determined by radioimmunoassay.We found that after exposure to hypoxia for 1h,4h,8h,12h,24h,HO-1 mRNA increased by 3%,4%,17%,31%,36% as compared with that in control group respectively(P