Mechanism of Duhuo Jisheng Decoction based on bioinformatics and network pharmacology in treating osteoporosis of kidney Yin deficiency

Objective:To study the active ingredients, target and mechanism of Duhuo JiSheng Decoction for the treatment of osteoporosis by bioinformatics analysis and network pharmacology. Methods: The gene expression profile of GSE56116 was acquired through the NCBI GEO database, and the software R was used for differential analysis. The Chinese medicine system pharmacology technology platform (TCMSP) was used to retrieve the chemical components, target genes of Duhuo, Fangfeng, Xixin, and Sangjisheng.The differential genes acquired from GSE56116 were intersected and analyzed by GO enrichment analysis and KEGG pathway to study the mechanism of Duhuo JiSheng Decoction.Results: A total of 33 active ingredients and 9 differential genes of Duhuo JiSheng Decoction for the treatment of Shengyin deficiency osteoporosis were screened. GO function enrichment analysis focuses on the regulation of intracellular signal transduction, calcium-mediated signaling, and steroid hormone mediated signaling. KEGG analysis showed that it was mainly concentrated in signal pathways such as P53 signal pathway, TNF signal pathway, and IL-17 signal pathway.Conclusion: Through bioinformatics and network pharmacological analysis, Duhuo JiShenng Decoction may exert therapeutic effects by acting on AR, IGFBP3, and FOS target genes.