VP3对鼻咽癌细胞CNE-2细胞株的体外杀伤效应
目的：体外实验观察VP3对鼻咽癌CNE-2细胞株的杀伤效应。方法：构建质粒表达载体pcDNA3．1（-）CMV．V3-His，KpnI／EcoR I酶切鉴定，测序分析VP3基因序列。脂质体法将pcDNA3．1（-）CMV．VP3-His和pcDNA3．1（-）-His分别瞬时转染鼻咽癌细胞CNE-2细胞株，Western印迹鉴定VP3基因的表达。四甲基偶氮唑盐（methylthiazoletetrazolium，MTT）法检测VP3对鼻咽癌细胞株CNE-2的杀伤效应。结果：酶切和测序分析证实重组质粒中包含完整的忡，基因的编码序列，Western印迹在转染细胞总蛋白中检测到该基因表达的16．03...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2006, Vol.31 (5), p.706-709 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
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| creator | 徐婧 邱元正 唐瑶云 田勇泉 肖献忠 赵素萍 |
| description | 目的：体外实验观察VP3对鼻咽癌CNE-2细胞株的杀伤效应。方法：构建质粒表达载体pcDNA3．1（-）CMV．V3-His，KpnI／EcoR I酶切鉴定，测序分析VP3基因序列。脂质体法将pcDNA3．1（-）CMV．VP3-His和pcDNA3．1（-）-His分别瞬时转染鼻咽癌细胞CNE-2细胞株，Western印迹鉴定VP3基因的表达。四甲基偶氮唑盐（methylthiazoletetrazolium，MTT）法检测VP3对鼻咽癌细胞株CNE-2的杀伤效应。结果：酶切和测序分析证实重组质粒中包含完整的忡，基因的编码序列，Western印迹在转染细胞总蛋白中检测到该基因表达的16．03kD的蛋白。表达VP3基因的CNE-2细胞生长受到抑制，而不表达忡；基因的CNE-2细胞生长未受到抑制。结论：VP3可杀伤鼻咽癌细胞CNE-2细胞株。 |
| doi_str_mv | 10.3321/j.issn:1672-7347.2006.05.019 |
| format | Article |
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Yi xue ban</title><addtitle>Journal of Central South University (Medical Sciences) Journal of Central South University (Medical Sciences)</addtitle><description>目的：体外实验观察VP3对鼻咽癌CNE-2细胞株的杀伤效应。方法：构建质粒表达载体pcDNA3．1（-）CMV．V3-His，KpnI／EcoR I酶切鉴定，测序分析VP3基因序列。脂质体法将pcDNA3．1（-）CMV．VP3-His和pcDNA3．1（-）-His分别瞬时转染鼻咽癌细胞CNE-2细胞株，Western印迹鉴定VP3基因的表达。四甲基偶氮唑盐（methylthiazoletetrazolium，MTT）法检测VP3对鼻咽癌细胞株CNE-2的杀伤效应。结果：酶切和测序分析证实重组质粒中包含完整的忡，基因的编码序列，Western印迹在转染细胞总蛋白中检测到该基因表达的16．03kD的蛋白。表达VP3基因的CNE-2细胞生长受到抑制，而不表达忡；基因的CNE-2细胞生长未受到抑制。结论：VP3可杀伤鼻咽癌细胞CNE-2细胞株。</description><subject>VP3</subject><subject>杀伤</subject><subject>鼻咽癌</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNo9j0tLw0AAhPegYKn9EyJ4Stz3Zr1JqA8o6qF4DbubbJu2pGgQ9SYo4sGTL1BQPPTkxbO2_TdNov_CSMTTDMPHDAPAMoIuIRit9tw4TZM1xAV2BKHCxRByFzIXIjkHav_5AmikaawhlFIS7oka8Pb3SPb-8T0ZZzfT4vG6GF9-nb_4O00HVzZ_fSueLmbT22z0kD-fzSaj_P4q-7xbBPNWDdKo8ad10N5otv0tp7W7ue2vtxzDpHSwRJ7wBDc8lBGxnBsCEbcaISyUQUZjRbUlDFMrIFNCh9p4EFLFI0MZRaQOVqraY5VYlXSC3vDoMCkHg25y2g9Pfo9CVt4syaWKNN1h0jmIS1Yr07fxIAowQYxRLMkPjeRmOQ</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>徐婧 邱元正 唐瑶云 田勇泉 肖献忠 赵素萍</creator><general>中南大学湘雅医院耳鼻咽喉科,长沙,410078%中南大学湘雅医学院病理生理学教研室,长沙,410078</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2006</creationdate><title>VP3对鼻咽癌细胞CNE-2细胞株的体外杀伤效应</title><author>徐婧 邱元正 唐瑶云 田勇泉 肖献忠 赵素萍</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c599-29187876c6d9e3f66c3016fb1127ac1cb2a4bf3524f705a7bdbc8004a6ec45413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2006</creationdate><topic>VP3</topic><topic>杀伤</topic><topic>鼻咽癌</topic><toplevel>online_resources</toplevel><creatorcontrib>徐婧 邱元正 唐瑶云 田勇泉 肖献忠 赵素萍</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>徐婧 邱元正 唐瑶云 田勇泉 肖献忠 赵素萍</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VP3对鼻咽癌细胞CNE-2细胞株的体外杀伤效应</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Journal of Central South University (Medical Sciences) Journal of Central South University (Medical Sciences)</addtitle><date>2006</date><risdate>2006</risdate><volume>31</volume><issue>5</issue><spage>706</spage><epage>709</epage><pages>706-709</pages><issn>1672-7347</issn><abstract>目的：体外实验观察VP3对鼻咽癌CNE-2细胞株的杀伤效应。方法：构建质粒表达载体pcDNA3．1（-）CMV．V3-His，KpnI／EcoR I酶切鉴定，测序分析VP3基因序列。脂质体法将pcDNA3．1（-）CMV．VP3-His和pcDNA3．1（-）-His分别瞬时转染鼻咽癌细胞CNE-2细胞株，Western印迹鉴定VP3基因的表达。四甲基偶氮唑盐（methylthiazoletetrazolium，MTT）法检测VP3对鼻咽癌细胞株CNE-2的杀伤效应。结果：酶切和测序分析证实重组质粒中包含完整的忡，基因的编码序列，Western印迹在转染细胞总蛋白中检测到该基因表达的16．03kD的蛋白。表达VP3基因的CNE-2细胞生长受到抑制，而不表达忡；基因的CNE-2细胞生长未受到抑制。结论：VP3可杀伤鼻咽癌细胞CNE-2细胞株。</abstract><pub>中南大学湘雅医院耳鼻咽喉科,长沙,410078%中南大学湘雅医学院病理生理学教研室,长沙,410078</pub><doi>10.3321/j.issn:1672-7347.2006.05.019</doi><tpages>4</tpages></addata></record> |
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2006, Vol.31 (5), p.706-709 |
| issn | 1672-7347 |
| language | chi |
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| source | Alma/SFX Local Collection |
| subjects | VP3 杀伤 鼻咽癌 |
| title | VP3对鼻咽癌细胞CNE-2细胞株的体外杀伤效应 |
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