华中神农箭竹更新幼龄地下茎伸长规律研究

Denaturing gradient gel electrophoresis （DGGE） was used to study the ef- fects of different soil additives, Le. control soil ＋ Glomus mossea-2 （G.M）, organic substrate, organic substrate ＋ G.M, on microbial molecular diversity in sweet pepper rhizosphere under protected cultivation. Results showed that following the inoculation of G.M, the total number of bacterial species increased, with the appearance and disappearance of some bacterial species. Some bacteria, though detected in all treatments, showed increased cell/clone numbers with the addition of arbuscular myc- orrhizal fungus G.M. The organic substrate favored the growth and propagation of some bacteria and was advantageous to the establishment of some dominant micro- bial flora. Resulted from the similarity analyses of the DGGE profile, it was obvious that organic substrate had more influence on the diversity of rhizosphere microbial flora than the inoculation of arbuscular mycorrhizal fungus G.M. Meanwhile, bacterial diversity in the control soil inoculated with G.M was higher than the organic soil. It was thus concluded that the inoculation of G.M changed the structure of microbial population in sweet pepper rhizosphere and increased the similarity of the rhizo- sphere bacterial species.