Preparation and preliminary biological evaluation of ^99mTc-ANMdU

Technetium-^99m-labeled-5- {2-sulfanylethyl- [2-（2-sulfanylethylamino）acetyl]amino }-methyl-2＇-deoxy- uridine （^99mTc-ANMdU） was reported. The precursor ANMdU was synthesized by six-step reactions and all intermediates were verified with MS and 1HNMR. Using SnCl2 as reducing agent, a labeling reaction was carried out at 100℃ for 30 min. The radiochemical purity of the ^99mTc-ANMdU was 96.68%. Partition coefficients were 0.92 and 0.70 at pH 7.0 and 7.4 of the phosphate buffer saline, respectively. Biodistribution of ^99mTc-ANMdU in normal mice showed that the initial uptake of ^99mTc-ANMdU in vivo and the clearance was rapid.