Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP

Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP

Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50...

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Veröffentlicht in:国际转化医学杂志（英文版） 2015, Vol.3 (1), p.28-32
Hauptverfasser: CAI Lei, RAO Xiao-hui, SU Qi, QIN Jia-sheng, CAI Li-quan, HONG He, WANG Kang-hua
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container_title 国际转化医学杂志（英文版）
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creator CAI Lei
RAO Xiao-hui
SU Qi
QIN Jia-sheng
CAI Li-quan
HONG He
WANG Kang-hua
description Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP.Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P
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In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP.Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P&lt;0.01) and the level of serum GP73 and AFP-L3 (%) in Non-PHC group were higher than those in healthy group (P&lt;0.01). ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(%) in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78 μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0vs. 71.7 and 60.0,P&lt;0.05) but the speciifcity was similar between single detection and joint detection (P&gt;0.05). The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was signiifcant differences in negative predictive value (P&lt;0.05) but no difference in positive predictive value (P&gt;0.05). The overall response rate of joint detection of GP73 and AFP-L3 was higher than single detection of them (P&lt;0.05). Conclusion:single detection of GP73 and AFP-L3 (%) were similar in the sensitivity and speciifcity in diagnosis of PHC patients with low concentration of AFP, which can be auxiliary diagnosis for PHC patients and joint detection of them is more reliable to diagnose PHC.</description><identifier>ISSN: 2227-6394</identifier><identifier>EISSN: 2227-6394</identifier><identifier>DOI: 10.11910/2227-6394.2015.03.01.06</identifier><language>eng</language><publisher>Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, 510280, China%Department of Hepatobiliary Surgery, Huizhou Municipal Central Hospital, Huizhou, 516001, China%Radiology Department, Qinhuangdao Chinese Medical Hospital, Qinhuangdao, 066002, China</publisher><ispartof>国际转化医学杂志（英文版）, 2015, Vol.3 (1), p.28-32</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/gjzhyxzz-e/gjzhyxzz-e.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>CAI Lei</creatorcontrib><creatorcontrib>RAO Xiao-hui</creatorcontrib><creatorcontrib>SU Qi</creatorcontrib><creatorcontrib>QIN Jia-sheng</creatorcontrib><creatorcontrib>CAI Li-quan</creatorcontrib><creatorcontrib>HONG He</creatorcontrib><creatorcontrib>WANG Kang-hua</creatorcontrib><title>Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP</title><title>国际转化医学杂志（英文版）</title><description>Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP.Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P&lt;0.01) and the level of serum GP73 and AFP-L3 (%) in Non-PHC group were higher than those in healthy group (P&lt;0.01). ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(%) in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78 μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0vs. 71.7 and 60.0,P&lt;0.05) but the speciifcity was similar between single detection and joint detection (P&gt;0.05). The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was signiifcant differences in negative predictive value (P&lt;0.05) but no difference in positive predictive value (P&gt;0.05). The overall response rate of joint detection of GP73 and AFP-L3 was higher than single detection of them (P&lt;0.05). Conclusion:single detection of GP73 and AFP-L3 (%) were similar in the sensitivity and speciifcity in diagnosis of PHC patients with low concentration of AFP, which can be auxiliary diagnosis for PHC patients and joint detection of them is more reliable to diagnose PHC.</description><issn>2227-6394</issn><issn>2227-6394</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpNUEtPAjEQbowmEuQ_9G52nbbQbo9kEdBsIgc9k6G0UAKt7hZX-fUu8RFP8833mmQIoQxyxjSDO865yqTQw5wDG-UgcmA5yAvS-1Mu_-FrMmiaHUDnLaRWskfeJh43ITbJG_qO-6Ol0dHH6EOiE5usST6GMzVbKEExrOl4usgqQX2gi9ofsP6kc_uK53iJtfEhHpC2Pm1pFVtaxmBsSDX-1nTpG3LlcN_Ywc_sk5fp_XM5z6qn2UM5rrKWcZayQumRdOBYwbQsjJPaoRtqYVe4ZlJypxUMC2Crzr0SWjjk1oxEAcapbrGiT26_e1sMDsNmuYvHOnQXl5vdafv5cTot7flpwACU-AIKh1_-</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>CAI Lei</creator><creator>RAO Xiao-hui</creator><creator>SU Qi</creator><creator>QIN Jia-sheng</creator><creator>CAI Li-quan</creator><creator>HONG He</creator><creator>WANG Kang-hua</creator><general>Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, 510280, China%Department of Hepatobiliary Surgery, Huizhou Municipal Central Hospital, Huizhou, 516001, China%Radiology Department, Qinhuangdao Chinese Medical Hospital, Qinhuangdao, 066002, China</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2015</creationdate><title>Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP</title><author>CAI Lei ; RAO Xiao-hui ; SU Qi ; QIN Jia-sheng ; CAI Li-quan ; HONG He ; WANG Kang-hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-w121t-87956f0f181968cf69faf493ebad1662f9704801b121b393fa2ec5380cf73fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CAI Lei</creatorcontrib><creatorcontrib>RAO Xiao-hui</creatorcontrib><creatorcontrib>SU Qi</creatorcontrib><creatorcontrib>QIN Jia-sheng</creatorcontrib><creatorcontrib>CAI Li-quan</creatorcontrib><creatorcontrib>HONG He</creatorcontrib><creatorcontrib>WANG Kang-hua</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>国际转化医学杂志（英文版）</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CAI Lei</au><au>RAO Xiao-hui</au><au>SU Qi</au><au>QIN Jia-sheng</au><au>CAI Li-quan</au><au>HONG He</au><au>WANG Kang-hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP</atitle><jtitle>国际转化医学杂志（英文版）</jtitle><date>2015</date><risdate>2015</risdate><volume>3</volume><issue>1</issue><spage>28</spage><epage>32</epage><pages>28-32</pages><issn>2227-6394</issn><eissn>2227-6394</eissn><abstract>Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP.Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P&lt;0.01) and the level of serum GP73 and AFP-L3 (%) in Non-PHC group were higher than those in healthy group (P&lt;0.01). ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(%) in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78 μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0vs. 71.7 and 60.0,P&lt;0.05) but the speciifcity was similar between single detection and joint detection (P&gt;0.05). The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was signiifcant differences in negative predictive value (P&lt;0.05) but no difference in positive predictive value (P&gt;0.05). The overall response rate of joint detection of GP73 and AFP-L3 was higher than single detection of them (P&lt;0.05). Conclusion:single detection of GP73 and AFP-L3 (%) were similar in the sensitivity and speciifcity in diagnosis of PHC patients with low concentration of AFP, which can be auxiliary diagnosis for PHC patients and joint detection of them is more reliable to diagnose PHC.</abstract><pub>Second Department of Hepatobiliary Surgery, Zhujiang Hospital, State Key Laboratory of Organ Failure Research, Co-Innovation Center for Organ Failure Research, Southern Medical University, Guangzhou, 510280, China%Department of Hepatobiliary Surgery, Huizhou Municipal Central Hospital, Huizhou, 516001, China%Radiology Department, Qinhuangdao Chinese Medical Hospital, Qinhuangdao, 066002, China</pub><doi>10.11910/2227-6394.2015.03.01.06</doi><tpages>5</tpages></addata></record>
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title Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP
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