Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP

Objective:To explore the applicative value of serum Golgi protein (GP73) and alpha-protein-variant (AFP-L3) in the diagnosis of patients with primary hepatic carcinoma (PHC).Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1~108.0 μg/L)) and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer). In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA) trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP.Results:The levels of serum GP73 and AFP-L3(%) in PHC group were signiifcantly higher than the other 2 groups (P