Effect of Fluvastatin on Oxidation-reduction Function and ICAM-1 Expression in Pocine Carotid Artery Endothelial Cells Dealt with Hypoxia/Reoxygenation
Objective:To explore the protective function of lfuvastatinon endothelial cells in an ischemia-reperfusion process. Methods:Pocine carotid artery endothelial cells (PCAEC) were cultured, grown together with different concentrations of fluvastatin (0.1 μmol/L, 0.2 μmol/L, 0.5 μmol/L, 1.0 μmol/L) for...
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Veröffentlicht in: | 国际转化医学杂志(英文版) 2014-12, Vol.2 (4), p.429-433 |
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Sprache: | eng |
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Zusammenfassung: | Objective:To explore the protective function of lfuvastatinon endothelial cells in an ischemia-reperfusion process. Methods:Pocine carotid artery endothelial cells (PCAEC) were cultured, grown together with different concentrations of fluvastatin (0.1 μmol/L, 0.2 μmol/L, 0.5 μmol/L, 1.0 μmol/L) for 44 h, and then divided into normal control group, different concentrations of lfuvastatin groups and H/R group. Serum immunology and cell immunochemistry were used to detect the levels of methyl thiazolyl tetrazolium (MTT), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), superoxide dismutase (SOD) and intercellular adhesion molecule-1 (ICAM-1) after 1-h hypoxia and 3-h reoxygenation. The effect of fluvastatin on oxidation-reduction function and ICAM-1 expression in PCAEC dealt with hypoxia/reoxygenation (H/R) was observed. Results:There was signiifcant difference regarding the cell viability between H/R group intervened by 0.5 μmol/L of lfuvastatin and simple H/R group (P=0.01). H/R could obviously decrease SOD activity in culture cells, and the generated MDA was conspicuously higher by comparison to lfuvastatin group and normal control group (P=0.001). Signiifcant differences were presented regarding GSH-PX level between normal control group, lfuvastatin group and H/R group (P=0.002). Additionally, ICAM-1 cell immunochemical staining showed marked differences among each group (P=0.018). Conclusion: Proper concentration of lfuvastatin can protect H/R endothelial cells. |
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ISSN: | 2227-6394 2227-6394 |
DOI: | 10.11910/2227-6394.2014.02.04.01 |