Design and Synthesis of 3-Triazolo-coumarins and Their Applications in Scavenging Radicals and Protecting DNA

In this study, we synthesized a series of 3-triazolo-coumarins and evaluated their antioxidant activities respectively by two methods： trapping 2,2＇-azinobis（3-ethylbenzothiazoline-6-sulfonate） cationic radical（ABTS＋＋） and oxidation of DNA which was induced by Cu2＋/glutathione（GSH）, ·OH and 2,2＇-azobis（2-amidinopropane hydrochloride）（AAPH）. Among the nine 3-triazolo-coumarins, compounds 6c and 6f-6i were synthesized for the first time, which exhibited the capability of terminating radical propagation-chains in oxidation of DNA induced by AAPH. In this study, we found that phenethylamine moiety, hydroxyl and ortho-methoxy are the key groups to en- hance the antioxidant activities of compounds.