LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
A rapid and sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard（IS）, tramadol, were separated on a Zo...
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| Veröffentlicht in: | Chemical research in Chinese universities 2014-12, Vol.30 (6), p.900-904 |
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| creator | Shi, Meiyun Yin, Lei Cai, Lanlan Wang, Can Liu, Xidong Zhao, Sen Sun, Yantong Fawcett, Paul J. Zhao, Limei Yang, Yan Gu, Jingkai |
| description | A rapid and sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard（IS）, tramadol, were separated on a Zorbax XDB C8 column using ace- tonitrile/1%（volume fraction） formic acid（volume ratio 35：65, pH=2.5） as mobile phase at a flow rate of 1.0 mL/min with a 1 ： 1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2（quantifier） and m/z 576.3→432.2（qualifier） and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 rain, respectively. The assay was linear over the concentration range of 0.1-100 gg/mL for 20 μL of human plasma only with intra- and inter-day preci- sions（expressed as the relative standard deviation） of less than 6.62% and accuracies（as relative error） of ＋1.31%. The method was applied to the pharmacokinetic study of a l-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers（n=6）. |
| doi_str_mv | 10.1007/s40242-014-4116-9 |
| format | Article |
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After the protein precipitation of sample with acetonitrile, the analyte and internal standard（IS）, tramadol, were separated on a Zorbax XDB C8 column using ace- tonitrile/1%（volume fraction） formic acid（volume ratio 35：65, pH=2.5） as mobile phase at a flow rate of 1.0 mL/min with a 1 ： 1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2（quantifier） and m/z 576.3→432.2（qualifier） and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 rain, respectively. The assay was linear over the concentration range of 0.1-100 gg/mL for 20 μL of human plasma only with intra- and inter-day preci- sions（expressed as the relative standard deviation） of less than 6.62% and accuracies（as relative error） of ＋1.31%. The method was applied to the pharmacokinetic study of a l-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers（n=6）.</description><identifier>ISSN: 1005-9040</identifier><identifier>EISSN: 2210-3171</identifier><identifier>DOI: 10.1007/s40242-014-4116-9</identifier><language>eng</language><publisher>Heidelberg: Jilin University and The Editorial Department of Chemical Research in Chinese Universities</publisher><subject>Analytical Chemistry ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Inorganic Chemistry ; LC-MS ; Organic Chemistry ; Physical Chemistry ; S方法 ; 人血浆 ; 头孢 ; 应用 ; 相对标准偏差 ; 药代动力学 ; 蛋白质沉淀</subject><ispartof>Chemical research in Chinese universities, 2014-12, Vol.30 (6), p.900-904</ispartof><rights>Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2014</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-b969a69a0f81b9fb979fdf424e4a17eb3d4e199eda722cf7bdd14b31236064b83</citedby><cites>FETCH-LOGICAL-c349t-b969a69a0f81b9fb979fdf424e4a17eb3d4e199eda722cf7bdd14b31236064b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86071X/86071X.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40242-014-4116-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40242-014-4116-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Shi, Meiyun</creatorcontrib><creatorcontrib>Yin, Lei</creatorcontrib><creatorcontrib>Cai, Lanlan</creatorcontrib><creatorcontrib>Wang, Can</creatorcontrib><creatorcontrib>Liu, Xidong</creatorcontrib><creatorcontrib>Zhao, Sen</creatorcontrib><creatorcontrib>Sun, Yantong</creatorcontrib><creatorcontrib>Fawcett, Paul J.</creatorcontrib><creatorcontrib>Zhao, Limei</creatorcontrib><creatorcontrib>Yang, Yan</creatorcontrib><creatorcontrib>Gu, Jingkai</creatorcontrib><title>LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study</title><title>Chemical research in Chinese universities</title><addtitle>Chem. Res. Chin. Univ</addtitle><addtitle>Chemical Research in Chinese University</addtitle><description>A rapid and sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard（IS）, tramadol, were separated on a Zorbax XDB C8 column using ace- tonitrile/1%（volume fraction） formic acid（volume ratio 35：65, pH=2.5） as mobile phase at a flow rate of 1.0 mL/min with a 1 ： 1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2（quantifier） and m/z 576.3→432.2（qualifier） and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 rain, respectively. The assay was linear over the concentration range of 0.1-100 gg/mL for 20 μL of human plasma only with intra- and inter-day preci- sions（expressed as the relative standard deviation） of less than 6.62% and accuracies（as relative error） of ＋1.31%. The method was applied to the pharmacokinetic study of a l-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers（n=6）.</description><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Inorganic Chemistry</subject><subject>LC-MS</subject><subject>Organic Chemistry</subject><subject>Physical Chemistry</subject><subject>S方法</subject><subject>人血浆</subject><subject>头孢</subject><subject>应用</subject><subject>相对标准偏差</subject><subject>药代动力学</subject><subject>蛋白质沉淀</subject><issn>1005-9040</issn><issn>2210-3171</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEURYMoWD9-gLvgzkVsvpoxSylqCy1WquuQmSSdqW1SJxls_72REd0JD95b3PMuHACuCL4lGBfDyDHlFGHCESdEIHkEBpQSjBgpyDEY5NAISczxKTiLcY0xk0LwAfCzMZovh_MlnNtUBwNdaGGqLXzptE9N0qkJHgYHx9aFZJP2sPFw0m3zsdjouNVQewOnKcL73W7TVD2QAlzUut3qKrw33qamgsvUmcMFOHF6E-3lzz4Hb48Pr-MJmj0_Tcf3M1QxLhMqpZA6D3Z3pJSulIV0xnHKLdeksCUz3BIprdEFpZUrSmMILxmhTGDByzt2Dm76v5_aO-1Xah261udGtTL7fb0_rGlWhUW2krOkz1ZtiLG1Tu3aZqvbgyJYfctVvVyVCfUtV8nM0J6JOetXtv0r-A-6_imqg199ZO63SQhWjJgcFewLi2WIMQ</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Shi, Meiyun</creator><creator>Yin, Lei</creator><creator>Cai, Lanlan</creator><creator>Wang, Can</creator><creator>Liu, Xidong</creator><creator>Zhao, Sen</creator><creator>Sun, Yantong</creator><creator>Fawcett, Paul J.</creator><creator>Zhao, Limei</creator><creator>Yang, Yan</creator><creator>Gu, Jingkai</creator><general>Jilin University and The Editorial Department of Chemical Research in Chinese Universities</general><general>Clinical Pharmacology Center, Research Institute of Translational Medicine, the First Hospital of Jilin University,Changchun 130061, P.R.China%College of Life Science, Jilin University, Changchun 130012, P.R.China%School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P.R.China</general><general>College of Life Science, Jilin University, Changchun 130012, P.R.China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20141201</creationdate><title>LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study</title><author>Shi, Meiyun ; Yin, Lei ; Cai, Lanlan ; Wang, Can ; Liu, Xidong ; Zhao, Sen ; Sun, Yantong ; Fawcett, Paul J. ; Zhao, Limei ; Yang, Yan ; Gu, Jingkai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-b969a69a0f81b9fb979fdf424e4a17eb3d4e199eda722cf7bdd14b31236064b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Inorganic Chemistry</topic><topic>LC-MS</topic><topic>Organic Chemistry</topic><topic>Physical Chemistry</topic><topic>S方法</topic><topic>人血浆</topic><topic>头孢</topic><topic>应用</topic><topic>相对标准偏差</topic><topic>药代动力学</topic><topic>蛋白质沉淀</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Meiyun</creatorcontrib><creatorcontrib>Yin, Lei</creatorcontrib><creatorcontrib>Cai, Lanlan</creatorcontrib><creatorcontrib>Wang, Can</creatorcontrib><creatorcontrib>Liu, Xidong</creatorcontrib><creatorcontrib>Zhao, Sen</creatorcontrib><creatorcontrib>Sun, Yantong</creatorcontrib><creatorcontrib>Fawcett, Paul J.</creatorcontrib><creatorcontrib>Zhao, Limei</creatorcontrib><creatorcontrib>Yang, Yan</creatorcontrib><creatorcontrib>Gu, Jingkai</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chemical research in Chinese universities</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Meiyun</au><au>Yin, Lei</au><au>Cai, Lanlan</au><au>Wang, Can</au><au>Liu, Xidong</au><au>Zhao, Sen</au><au>Sun, Yantong</au><au>Fawcett, Paul J.</au><au>Zhao, Limei</au><au>Yang, Yan</au><au>Gu, Jingkai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study</atitle><jtitle>Chemical research in Chinese universities</jtitle><stitle>Chem. Res. Chin. Univ</stitle><addtitle>Chemical Research in Chinese University</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>30</volume><issue>6</issue><spage>900</spage><epage>904</epage><pages>900-904</pages><issn>1005-9040</issn><eissn>2210-3171</eissn><abstract>A rapid and sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard（IS）, tramadol, were separated on a Zorbax XDB C8 column using ace- tonitrile/1%（volume fraction） formic acid（volume ratio 35：65, pH=2.5） as mobile phase at a flow rate of 1.0 mL/min with a 1 ： 1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2（quantifier） and m/z 576.3→432.2（qualifier） and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 rain, respectively. The assay was linear over the concentration range of 0.1-100 gg/mL for 20 μL of human plasma only with intra- and inter-day preci- sions（expressed as the relative standard deviation） of less than 6.62% and accuracies（as relative error） of ＋1.31%. The method was applied to the pharmacokinetic study of a l-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers（n=6）.</abstract><cop>Heidelberg</cop><pub>Jilin University and The Editorial Department of Chemical Research in Chinese Universities</pub><doi>10.1007/s40242-014-4116-9</doi><tpages>5</tpages></addata></record> |
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| subjects | Analytical Chemistry Chemistry Chemistry and Materials Science Chemistry/Food Science Inorganic Chemistry LC-MS Organic Chemistry Physical Chemistry S方法 人血浆 头孢 应用 相对标准偏差 药代动力学 蛋白质沉淀 |
| title | LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study |
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