LC-MS/MS Method for the Quantitation of Cefotetan in Human Plasma and Its Application to Pharmacokinetic Study
A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zo...
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Veröffentlicht in: | Chemical research in Chinese universities 2014-12, Vol.30 (6), p.900-904 |
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Sprache: | eng |
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Zusammenfassung: | A rapid and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the de- termination of cefotetan in human plasma was developed and validated. After the protein precipitation of sample with acetonitrile, the analyte and internal standard(IS), tramadol, were separated on a Zorbax XDB C8 column using ace- tonitrile/1%(volume fraction) formic acid(volume ratio 35:65, pH=2.5) as mobile phase at a flow rate of 1.0 mL/min with a 1 : 1 split. The detection was performed by electrospray ionization with positive ion mode, followed by multiple reaction monitoring of the transitions for cefotetan at m/z 576.3→460.2(quantifier) and m/z 576.3→432.2(qualifier) and for IS at m/z 264.1→58.1. Cefotetan and IS were eluted at 1.86 and 1.87 rain, respectively. The assay was linear over the concentration range of 0.1-100 gg/mL for 20 μL of human plasma only with intra- and inter-day preci- sions(expressed as the relative standard deviation) of less than 6.62% and accuracies(as relative error) of +1.31%. The method was applied to the pharmacokinetic study of a l-h intravenous infusion of 1.0 g of cefotetan disodium for human volunteers(n=6). |
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ISSN: | 1005-9040 2210-3171 |
DOI: | 10.1007/s40242-014-4116-9 |