Improvement of Properties of Pseudomonas sp. Lipase in Resolution of 2－Octanol

A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-oc-tanol in a solvent-free system. The E value was improved from 6. 64 to 120. 2. The lipase modified by the an-ionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na4P2O7 · H2O and Na4P2O7 can dramatically activate the modified enzyme.