Optimization of Adsorption Conditions of Papain on Cross-Linked Phage-Displayed Heptapeptide Matrix Using Response Surface Methodology

A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adsorption. Finally, the adsorption conditions were optimized by response surface methodology. The results indicated that the optimum adsorption conditions were determined as initial pH 6.9, temperature 40℃, and the adsorption capacity of CHM for papain was found to be 55.52 mg/g. Moreover, the papain was purified 78- fold in a single step determined by affinity precipitation. More than 87 % of the adsorbed papain was desorbed using the eluent solventtriethylarnine at pH 7.4. The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.