Development of Molecular Marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

Development of Molecular Marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit t...

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Veröffentlicht in:The journal of Northeast Agricultural University 2012-12, Vol.19 (4), p.30-36
Hauptverfasser: Ning, Li, Jing-bin, Jiang, Jing-fu, Li, Xiang-yang, Xu
Format: Artikel
Sprache:eng
Schlagworte:
Cf-10 resistant gene
Cladosporium fulvum
molecular marker
tomato
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Zusammenfassung:The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.
ISSN:1006-8104
DOI:10.1016/S1006-8104(13)60047-1