Study on the Tissue Culture of Coleus blumei
This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA). The optimal conditions of callus induction from explants were ach...
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Veröffentlicht in: | The journal of Northeast Agricultural University 2008-03, Vol.15 (1), p.14-17 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA). The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA (2.0 mg· L^-1) and NAA (1.0 mg· L^-1). Shoot tips were induced on the medium containing 6-BA (4.0 mg·L^-1) and NAA (0.5 mg·L^-1). The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA (0.1 mg·L^-1). |
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ISSN: | 1006-8104 |