EGF mRNA Expression in Pig Ovary

Epidermal growth factor(EGF)is known to enhance oocyte maturation,embryo development and implan-tation in many species To study the rules of EGF in pig ovary,the level of EGFmRNA activity was measured by RT-PCR technique.A strong EGF transcripts band was detected in the follicles and oocytes.The expres-sion of EGF is strongest in the small follicle or oocyte from small follicle.A EGF transcripts band could be detectable in the granulose cell.The expression of EGF in the granulosa cell was lower than that in the oocyte.Also,the expression of EGF in the granulosa cell from the small follicle is stronger than in another.These results suggest EGF has important roles in the pig follicular development by autocrine/paracrine fash-ion.