Echinococcus Granulosus: Suitable in vitro Protoscolices Culture Density

The present study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was a...

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Veröffentlicht in:Biomedical and environmental sciences 2013-11, Vol.26 (11), p.912-915
Hauptverfasser: LIU, Cong Shan, ZHANG, Hao Bing, YIN, Jian Hai, JIANG, Bin, HAN, Xiu Min
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Sprache:eng
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Zusammenfassung:The present study is to determine the suitable protoscolices (PSCs) density for long-time culturing in vitro. The PSCs were divided into eight groups with different densities and the viability tests were carried out with 0.1% methylene blue staining. Then the infection ability of cultured PSCs was assessed by the mean cyst weight of mice inoculated intraperitoneally with PSCs after 8 months post-infection.
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2013.020