Microbial Degradation of Quinoline： Kinetics Study With Burkholderia picekttii

Objective To investigate the kinetics of quinoline biodegradation by Burkholderia pickttii,a Gram negative rod-shaped aerobe, isolated in our laboratory.Methods HPLC (Hewlett-Packard model 5050 with an UV detector) was used for the analysis of quinoline concentration. GC/MS method was used to identify the intermediate metabolites of quinoline degradation. Results The biodegradation of quinoline was inhibited by quinoline at a high concentration, and the degradation process could be described by the Haldane model. The kinetic parameters based on Haldane substrate inhibition were cvaluatcd.The values were Vmax=0.44h^-1,Ks=166.7mg/L,Ki= 650mg/L,respectively The quinoline concentration to avoid substrate inhibition was inferred theoretically and determined to be 329mg/L.Conclusion The biodegradation of quinoline conforms to the Haldane inhibition model and the main intermediate metabolite of qulnoline biodegradation is 2-hydroxy-quinoline.