Development of a bioassay to test the possible role of thiamine disturbances as a mechanism behind pollution-induced reproductive failures in birds

A test system was developed to examine the effects of environmental contaminants on thiamine homeostasis in bird embryos. This system employs fresh chicken egg yolk lipids as a vehicle for use in egg injection studies. Furazolidone, an antibiotic suspected to interfere with thiamine metabolism, was used as a positive control to evaluate the utility of the test system. It was determined that fresh chicken egg yolk lipids were preferable over chemical vehicles as it resulted in lower mortality rates (16␟ersus 23-62€and did not induce any observable effects in the embryo. Injection of 1 mg/egg of furazolidone at day 0 of development resulted in decreased respiration followed by death, with mortality rates being twice as high as in carrier controls. In addition, transketolase activity, which was measured as an indicator of thiamine availability in the body, was decreased 25␒n brains of 19-day-old embryos. This mechanism may be of importance for effects of environmental contaminants in wild bird populations.