Sephadex filtration as successful alternative to density-gradient centrifugation procedures for ram sperm selection with improved kinetics

Sephadex filtration as successful alternative to density-gradient centrifugation procedures for ram sperm selection with improved kinetics

Density-gradients centrifugation (DGC) and filtration columns (FC) are used to separate deformed or dead sperm, debris, and other cells that may negatively affect the fertilizing capacity of sperm in fresh, chilled and frozen/thawed semen. The present study was conducted to evaluate the suitability...

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Veröffentlicht in:Animal reproduction science 2018-05, Vol.192, p.261-270
Hauptverfasser: Galarza, D.A., López-Sebastián, A., Woelders, H., Blesbois, E., Santiago-Moreno, J.
Format: Artikel
Sprache:eng
Schlagworte:
Centrifugation
Computer Science
Filtration
Gradients
Life Sciences
Selection
Sperm
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Zusammenfassung:Density-gradients centrifugation (DGC) and filtration columns (FC) are used to separate deformed or dead sperm, debris, and other cells that may negatively affect the fertilizing capacity of sperm in fresh, chilled and frozen/thawed semen. The present study was conducted to evaluate the suitability of DGC (BoviPure®, Percoll® and Accudenz®) and FC (Sephadex G-15®) sperm selection procedures for fresh-extended and cold-stored ram semen by assessment of post-treatment sperm quality variables. Twenty normospermic ejaculates from ten adult Merino rams were used. Sperm concentration of recovered cells was greater (P 
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2018.03.022