Dynamics of CMY-2 producing E. coli in a broiler parent flock

•A sharp decrease of CMY-2-E. coli in a broiler parent flock was observed.•CMY-2-E. coli persisted in the environment of the parent flock.•In absence of antibiotics CMY-2 on plasmid IncA/C may have a selective disadvantage. Extended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pA...

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Veröffentlicht in:Veterinary microbiology 2017-05, Vol.203, p.211-214
Hauptverfasser: Dame-Korevaar, Anita, Fischer, Egil A.J., Stegeman, Arjan, Mevius, Dik, van Essen-Zandbergen, Alieda, Velkers, Francisca, van der Goot, Jeanet
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Sprache:eng
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Zusammenfassung:•A sharp decrease of CMY-2-E. coli in a broiler parent flock was observed.•CMY-2-E. coli persisted in the environment of the parent flock.•In absence of antibiotics CMY-2 on plasmid IncA/C may have a selective disadvantage. Extended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pAmpC) producing bacteria are resistant to Extended Spectrum Cephalosporins (ESC), and are present in all levels of the broiler production chain. We determined the prevalence, concentration, and persistence of ESBL/pAmpC-Escherichia coli in a broiler parent flock during the rearing and laying period. One-day old chickens were housed in four separate pens. Until week 33 no antibiotics or coccidiostatics were used. During rearing 57 chickens in each pen (n=228), and in the laying period two groups of 33 chickens were individually sampled (n=66). Environmental samples were taken from week 16 onwards. ESBL/pAmpC-E. coli presence was determined by selective culturing. In the samples of week 16–19 the concentration of ESBL/pAmpC-E. coli was determined. All ESC-resistant isolates found were positive for pAmpC gene blaCMY-2 located on IncA/C plasmids, in several E. coli MLST types. CMY-2-E. coli prevalence decreased from 91% (95%CI 86–94%) at day 7 (week 1) to 0% (95%CI 0–5%) in week 21. However, CMY-2-E. coli remained present in the environmental samples during the whole study. CMY-2-E. coli concentration varied between detection limit (
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2017.03.024