Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization

Receptor kinases are essential for the cellular perception of signals. The classical model for activation of the receptor kinase involves dimerization, induced by the binding of the ligand. The mechanisms by which plant receptors transduce signals across the cell surface are largely unknown but plan...

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Hauptverfasser:	Hink, Mark A., de Vries, Sacco C., Visser, Antonie J. W. G.
Format:	Buchkapitel
Sprache:	eng
Schlagworte:	Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biochemie Biochemistry BRI1 Calibration Dimerization EPS-1 Fluorescence cross-correlation spectroscopy Fluorometry - methods Gene Expression Regulation, Plant Genetic Vectors - genetics Photon counting histogram Plasma membrane Protein Kinases - genetics Protein Kinases - metabolism Protein Multimerization Protoplast Protoplasts - metabolism Receptor kinase SERK1 SERK3 Spectrometry, Fluorescence - methods Transfection
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Beschreibung
Zusammenfassung:	Receptor kinases are essential for the cellular perception of signals. The classical model for activation of the receptor kinase involves dimerization, induced by the binding of the ligand. The mechanisms by which plant receptors transduce signals across the cell surface are largely unknown but plant receptors seem to dimerize as well. In this chapter, we describe two fluorescence fluctuation techniques, fluorescence cross-correlation spectroscopy and photon counting histogram analysis, to study the oligomerization state of receptor kinases in living plant cells in a quantitative manner.
ISSN:	1064-3745 1940-6029
DOI:	10.1007/978-1-61779-264-9_11