Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galalpha1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacteria...

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Veröffentlicht in:Organic & biomolecular chemistry 2010-01, Vol.8 (10), p.2425-2429
Hauptverfasser: Pera, Núria Parera, Kouki, Annika, Haataja, Sauli, Branderhorst, Hilbert M, Liskamp, Rob M J, Visser, Gerben M, Finne, Jukka, Pieters, Roland J
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphate - analysis
adhesion
Bacterial Proteins - metabolism
Carbohydrate Metabolism
Carbohydrates - chemistry
escherichia-coli
inhibition
Luminescent Measurements
Magnetics
nanoparticles
Particle Size
recognition
Streptococcus suis - isolation & purification
Streptococcus suis - metabolism
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Zusammenfassung:Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galalpha1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.
ISSN:1477-0520
1477-0539
DOI:10.1039/c000819b