A modified extraction protocol enables detection and quantification of celiac disease-related gluten proteins from wheat

The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, curre...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-04, Vol.877 (10), p.975-982
Hauptverfasser:	van den Broeck, Hetty C., America, Antoine H.P., Smulders, Marinus J.M., Bosch, Dirk, Hamer, Rob J., Gilissen, Ludovicus J.W.J., van der Meer, Ingrid M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Antigens, Plant - chemistry Antigens, Plant - isolation & purification Biological and medical sciences Celiac disease Celiac Disease - immunology Chemical Fractionation - methods endosperm proteins epitopes Flour - analysis foods Fundamental and applied biological sciences. Psychology gel-electrophoresis General pharmacology gliadin Gliadins Gluten protein extraction Glutenins Glutens - chemistry Glutens - isolation & purification high-molecular-weight Humans Immunoblotting Medical sciences Monoclonal antibodies monoclonal-antibody peptides Pharmacology. Drug treatments Plant Extracts - chemistry Plant Extracts - isolation & purification subunits Triticum - chemistry Triticum - immunology Wheat
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Beschreibung
Zusammenfassung:	The detection, analysis, and quantification of individual celiac disease (CD) immune responsive gluten proteins in wheat and related cereals (barley, rye) require an adequate and reliable extraction protocol. Because different types of gluten proteins behave differently in terms of solubility, currently different extraction protocols exist. The performance of various documented gluten extraction protocols is evaluated for specificity and completeness by gel electrophoresis (SDS-PAGE), immunoblotting and RIDASCREEN ® Gliadin competitive ELISA. Based on these results, an optimized, two-step extraction protocol has been developed.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2009.02.035