Loss of aphid transmissibility of plum pox virus isolates

Loss of aphid transmissibility of plum pox virus isolates

The aphid transmissibility of seven Plum pox virus (PPV) isolates and the amino acid sequences of their coat proteins were analysed. Two aphid transmissible isolates PPV-A and PPV-P contained the DAG amino triplet, while DAL or NAG replaced this triplet in the coat proteins of non-aphid transmissibl...

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Veröffentlicht in:Biotechnology, biotechnological equipment biotechnological equipment, 2002, Vol.16 (1), p.48-54
Hauptverfasser: Kamenova, I. (AgroBioInstitute, Kostinbrod (Bulgaria)), Lohuis, D, Peters, D
Format: Artikel
Sprache:eng
Schlagworte:
ACIDE AMINE
AMINO ACIDS
AMINOACIDOS
BRACHYCAUDUS
complete nucleotide-sequence
DISEASE TRANSMISSION
identification
MICROBIAL PROTEINS
MYZUS PERSICAE
PHORODON HUMULI
plant-viruses
PLUM POX POTYVIRUS
potyvirus
POTYVIRUS SHARKA DEL CIRUELO
POTYVIRUS SHARKA DU PRUNIER
protein gene
PROTEINAS MICROBIANAS
PROTEINE MICROBIENNE
purification
rna
strain
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
y helper component
yellow mosaic-virus
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Zusammenfassung:The aphid transmissibility of seven Plum pox virus (PPV) isolates and the amino acid sequences of their coat proteins were analysed. Two aphid transmissible isolates PPV-A and PPV-P contained the DAG amino triplet, while DAL or NAG replaced this triplet in the coat proteins of non-aphid transmissible isolates. In addition, a sequence of 15 or 17 amino acids was deleted in the coat proteins of PPV-P48, PPV-P45 and PPV-II/7. Helper components with Mr. of 52 kDa were successfully purified from N. benthamiana plants inoculated with aphid transmissible and non-aphid transmissible isolates. The helper components of two coat-proteins-defective isolates mediated aphid transmission of non- coat-protein defective isolates, indicating that the helper components were biologically active.
ISSN:1310-2818
1314-3530
DOI:10.1080/13102818.2002.10819155