Detection of Bovine Meat and Bone Meal in Animal Feed at a Level of 0.1

For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed s...

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Veröffentlicht in:	Journal of AOAC International 2006-11, Vol.89 (6), p.1443-1446
Hauptverfasser:	Aarts, H.J.M, Bouw, E.M, Buntjer, J.B, Lenstra, J.A, Raamsdonk, L.W.D. van
Format:	Artikel
Sprache:	eng
Schlagworte:	adulterated products Animal Feed - analysis animal proteins Animals Beef Biological and medical sciences Bone and Bones - chemistry Cattle cattle feeding Chickens detection DNA - chemistry Equipment Reuse evolution Feed and pet food industries feed composition feed contamination feeds Food and nutrition Food industries Fundamental and applied biological sciences. Psychology General aspects Identification and classification Meat - analysis meat and bone meal Methods of analysis, processing and quality control, regulation, standards Mutton Polymerase chain reaction Poultry poultry feeding Reverse Transcriptase Polymerase Chain Reaction Sheep species-specific dna
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Beschreibung
Zusammenfassung:	For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/89.6.1443