Affinity-tags are removed from Cladosporium fulvum effector proteins expressed in the tomato leaf apoplast

Cladosporium fulvum (syn. Passalora fulva) is a biotrophic fungal pathogen that causes leaf mould on tomato (Solanum esculentum). The fungus grows exclusively in the tomato leaf apoplast where it secretes several small (

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany 2006-02, Vol.57 (3), p.599-608
Hauptverfasser:	van Esse, H. Peter, Thomma, Bart P. H. J., van 't Klooster, John W., de Wit, Pierre J. G. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Amino Acid Sequence Antibodies Arabidopsis - genetics Arabidopsis thaliana Avirulence avr9 Biological and medical sciences c-myc Cell biochemistry Cell physiology cf-2-dependent disease resistance cf-4 locus cf-4-mediated resistance Cladosporium Cladosporium - genetics Cladosporium - metabolism Cladosporium - pathogenicity Cladosporium fulvum Cloning, Molecular elicitor extracellular FLAG Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - isolation & purification Fungal Proteins - metabolism Gels gene Genes Genetic vectors His identification Immunity, Innate Infections locus comprises Lycopersicon esculentum Lycopersicon esculentum - anatomy & histology Lycopersicon esculentum - genetics Lycopersicon esculentum - microbiology Molecular Sequence Data Passalora pathogen Pathogens Pichia Plant interaction Plant Leaves - genetics Plant Leaves - microbiology Plant physiology and development Plants, Genetically Modified - metabolism Proteins Proteomics purification Recombinant Fusion Proteins - metabolism Research Papers Solanum StrepII TAP targeted proteomics
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Beschreibung
Zusammenfassung:	Cladosporium fulvum (syn. Passalora fulva) is a biotrophic fungal pathogen that causes leaf mould on tomato (Solanum esculentum). The fungus grows exclusively in the tomato leaf apoplast where it secretes several small (
ISSN:	0022-0957 1460-2431
DOI:	10.1093/jxb/erj044