Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address

The high‐throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self‐assembly concepts. DNA‐directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here,...

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Veröffentlicht in:Proteomics (Weinheim) 2006-05, Vol.6 (9), p.2650-2655
Hauptverfasser: Jongsma, Maarten A., Litjens, Ralph H. G. M.
Format: Artikel
Sprache:eng
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Zusammenfassung:The high‐throughput deposition of recombinant proteins on chips, beads or biosensor devices would be greatly facilitated by the implementation of self‐assembly concepts. DNA‐directed immobilization via conjugation of proteins to an oligonucleotide would be preeminently suited for this purpose. Here, we present a unique method to attach a single DNA address to proteins in one step during the purification from the E. coli lysate by fusion to human O6‐alkylguanine‐DNA‐alkyltransferase (SNAP‐tag™) and the Avitag. Use of the conjugates in converting a DNA chip into a protein chip by self assembly is demonstrated.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500654