Liquid chromatographic method to determine narasin in feedingstuffs and premixtures: development, validation, and interlaboratory study

A reversed-phase liquid chromatography (LC) method for narasin in feedingstuffs and premixtures was developed, validated, and interlaboratory studied. The extraction solvent was methanol-K2HPO4 solution (9 + 1, v/v). Narasin was detected at 600 nm after post-column derivatization with dimethylamino-...

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Veröffentlicht in:	Journal of AOAC International 2004-11, Vol.87 (6), p.1278-1286
Hauptverfasser:	Thalmann, A, Wagner, K, Tomassen, M, Driessen, J, Jong, J. de
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Animal Feed - analysis Biological and medical sciences Biological Assay broiler chickens Calibration coccidiostats Coccidiostats - analysis derivatization European Union extraction feed additives Feed and pet food industries Food industries Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Indicators and Reagents medicated feeds narasin Pyrans - analysis Reference Standards Reproducibility of Results reversed-phase liquid chromatography Solvents Spectrophotometry, Ultraviolet Ultrasonics
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Zusammenfassung:	A reversed-phase liquid chromatography (LC) method for narasin in feedingstuffs and premixtures was developed, validated, and interlaboratory studied. The extraction solvent was methanol-K2HPO4 solution (9 + 1, v/v). Narasin was detected at 600 nm after post-column derivatization with dimethylamino-benzaldehyde. Recovery was >90%. The repeatability (RSD(r)) in feed (20-140 mg/kg) ranged between 1.2 and 10.5%; the within-laboratory reproducibility (RSDR) ranged between 2.2 and 4.9%. The limit of determination was
ISSN:	1060-3271 1944-7922
DOI:	10.1093/jaoac/87.6.1278