Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium

Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands Correspondence Muriel Derrien muriel.derrien{at}wur.nl The diversity of mucin-degrading bacteria in the human intestine was investigated by combining culture and 16S rRNA-dependent approaches. A dominant bacterium, strain Muc T , was isolated by dilution to extinction of faeces in anaerobic medium containing gastric mucin as the sole carbon and nitrogen source. A pure culture was obtained using the anaerobic soft agar technique. Strain Muc T was a Gram-negative, strictly anaerobic, non-motile, non-spore-forming, oval-shaped bacterium that could grow singly and in pairs. When grown on mucin medium, cells produced a capsule and were found to aggregate. Strain Muc T could grow on a limited number of sugars, including N -acetylglucosamine, N -acetylgalactosamine and glucose, but only when a protein source was provided and with a lower growth rate and final density than on mucin. The G+C content of DNA from strain Muc T was 47·6 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the division Verrucomicrobia . The closest described relative of strain Muc T was Verrucomicrobium spinosum (92 % sequence similarity). Remarkably, the 16S rRNA gene sequence of strain Muc T showed 99 % similarity to three uncultured colonic bacteria. According to the data obtained in this work, strain Muc T represents a novel bacterium belonging to a new genus in subdivision 1 of the Verrucomicrobia ; the name Akkermansia muciniphila gen. nov., sp. nov. is proposed; the type strain is Muc T (=ATCC BAA-835 T =CIP 107961 T ). Abbreviations: DGGE, denaturing gradient gel electrophoresis; GI, gastrointestinal; MPN, most probable number Published online ahead of print on 14 May 2004 as DOI 10.1099/ijs.0.02873-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Akkermansia muciniphila Muc T is AY271254 .