Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans

A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 X 10(2) to 2.5 X 10(4) transformants per 10(6) viable...

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Veröffentlicht in:	FEMS microbiology letters 1994-11, Vol.124 (1), p.35-41
Hauptverfasser:	Moreno, M.A, Pascual, C, Gibello, A, Ferrer, S, Bos, C.J, Debets, A.J.M, Suarez, G
Format:	Artikel
Sprache:	eng
Schlagworte:	aflatoxins Arginine B Aspergillus - genetics Aspergillus nidulans - genetics Aspergillus nidulans autonomous replicating plasmid Aspergillus nidulellus Aspergillus parasiticus Autonomous replication clones DNA Replication DNA, Fungal - analysis Genetic transformation Laboratorium voor Erfelijkheidsleer Laboratory of Genetics Mitosis plasmids Plasmids - genetics Protoplasts recombinant DNA Transformation, Genetic
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Beschreibung
Zusammenfassung:	A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 X 10(2) to 2.5 X 10(4) transformants per 10(6) viable protoplasts and per microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1994.tb07258.x