4 '-Guanidinium-modified siRNA : a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

4 '-Guanidinium-modified siRNA : a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

We designed novel 4 '-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against...

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Hauptverfasser: Nawale, Ganesh N, Bahadorikhalili, Saeed, Sengupta, Pallabi, Kadekar, Sandeep, Chatterjee, Subhrangsu, Varghese, Oommen P
Format: Artikel
Sprache:eng
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
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Zusammenfassung:We designed novel 4 '-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in approximate to 4.9-fold more efficient knockdown than unmodified siRNA. Correction in: CHEMICAL COMMUNICATIONS, Volume: 55, Issue: 67, Pages: 10028-10028, DOI: 10.1039/c9cc90352f
DOI:10.1039/c9cc04141a