High level of expression of INGAP in bacterial and eukaryotic cells

High level of expression of INGAP in bacterial and eukaryotic cells

Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows cultured cells to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to the cells.

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Bibliographische Detailangaben
Hauptverfasser: Vinik, Aaron I, Pittenger, Gary L, Rafaeloff-Phail, Ronit, Barlow, Scott W
Format: Patent
Sprache:eng
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Zusammenfassung:Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows cultured cells to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to the cells.