Auxotrophic

Agrobacterium Agrobacterium Agrobacterium Agrobacterium Agrobacterium Agrobacterium AgrobacteriumAgrobacterium Auxotrophic and methods employing auxotrophic are provided. Auxotrophic may be used in a variety of methods including biologically containing comprising a transgene and transforming a plant...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ranch, Jerome P, Liebergesell, Matthias, Garnaat, Carl W, Huffman, Gary A
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Agrobacterium Agrobacterium Agrobacterium Agrobacterium Agrobacterium Agrobacterium AgrobacteriumAgrobacterium Auxotrophic and methods employing auxotrophic are provided. Auxotrophic may be used in a variety of methods including biologically containing comprising a transgene and transforming a plant cell without using an counter-selective agent. Transforming maize immature embryos using an auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic . Methods for producing an thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.