Selective terminal tagging of nucleic acids

Selective terminal tagging of nucleic acids

Methods are provided for adding a terminal sequence tag to nucleic acid molecules for use in RNA or DNA amplification. The tag introduced may be used as a primer binding site for subsequent amplification of the DNA molecule and/or sequencing of the DNA molecule and therefore provides means for ident...

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Bibliographische Detailangaben
1. Verfasser: Sooknanan, Roy R
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Methods are provided for adding a terminal sequence tag to nucleic acid molecules for use in RNA or DNA amplification. The tag introduced may be used as a primer binding site for subsequent amplification of the DNA molecule and/or sequencing of the DNA molecule and therefore provides means for identification and cloning of the 5′-end or the complete sequence of mRNAs.