Apparatus for performing a reaction in a droplet and method of using the same

An apparatus for processing a biological and/or chemical sample in a liquid droplet is provided. The apparatus includes a processing compartment, a base and at least one circumferential wall. The processing compartment is defined by at least a part of the base, at least a part of the circumferential wall and an inlet member. The inlet member is located on top of the processing compartment, and includes at least one droplet inlet channel, which extends through the inlet member and includes a contraction between the inlet opening of the droplet inlet channel to the environment and the outlet opening to the processing compartment. There is furthermore provided a method of processing a biological and/or chemical sample in a liquid droplet. The method includes disposing a medium into the processing compartment of the apparatus of the invention that is immiscible with the liquid droplet, so that the contraction is immersed in the medium. The droplet is disposed into the droplet inlet channel such that it is located below the contraction of the droplet inlet channel. A process is performed on the biological and/or chemical sample in the droplet.