Method of extracting chromatin fractions from intact cells

Methods are provided for isolation of chromatin fractions of nucleoproteins containing histone H1, H2A, H2B, H3 and H4 proteins and/or histone H1, H2A, H2B, H3 and/or H4 proteins, from intact cells. The methods preserve original patterns of covalent modifications of the histone proteins.

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Bibliographische Detailangaben
Hauptverfasser: Rodriguez-Collazo, Pedro, Leuba, Sanford Harrison, Zlatanova, Jordanka
Format: Patent
Sprache:eng
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Zusammenfassung:Methods are provided for isolation of chromatin fractions of nucleoproteins containing histone H1, H2A, H2B, H3 and H4 proteins and/or histone H1, H2A, H2B, H3 and/or H4 proteins, from intact cells. The methods preserve original patterns of covalent modifications of the histone proteins.