Polynucleotides for the detection of

E. coli E. coli E. coli E. coli E. coli E. coli E. coli E. coli E. coli Polynucleotide primers and probes for the specific detection of 0157:H7 and/or 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of 0157:H7 and/or 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of 0157:H7 and/or 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing 0157:H7 and/or 0157:NM which produces verotoxin from other strains. Kits comprising the primers and probes are also provided.