Plant transformation and selection

Agrobacterium Eucalyptus Eucalyptus The present invention relates to a genotype-independent method for transforming and selecting plant explants. The transformation method includes pre-culturing the explants in the presence of an inducer and exposing the transformed explants to a shoot regeneration media that accelerates shoot development. Plants generated from this transformation method are provided. In particular, methods for obtaining transgenic and pine cells and regenerating stably transformed and pine trees are provided. The invention also provides media, methods, and plasmids for selecting and regenerating plants, particularly forest trees.