Process for the observation of at least one sample region with a light raster microscope with linear sampling

Process for observing at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a...

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Bibliographische Detailangaben
Hauptverfasser: Engelmann, Ralf, Funk, Joerg-Michael, Steinert, Joerg, Zimmermann, Bernhard, Wilhelm, Stefan, Engel, Joerg, Meisel, Ulrich
Format: Patent
Sprache:eng
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Zusammenfassung:Process for observing at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a line and are detected simultaneously with a spatially resolving detector. At an angle to the plane of the relative movement, a second scanner is moved and an image acquisition takes place by coupling the movement of the first and second scanners and a three-dimensional sampling movement being done by the illumination of the sample. The second scanner is coupled to the movement of the first scanner such that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.