Paired end sequencing

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target n...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	Berka, Jan, Chen, Zhoutao, Egholm, Michael, Godwin, Brian C, Hutchison, Stephen Kyle, Leamon, John Harris, Sarkis, Gary James, Simons, Jan Fredrik
Format:	Patent
Sprache:	eng
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.