Primers and method of detecting bacteria

Escherichia, Salmonella Vibrio Staphylococcus aureus Bacillus cereus, The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the and genera, but when used in PCR under the same conditions,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Nakano, Shigeru, Kobayashi, Toru, Funabiki, Kenichi, Nagao, Yasuhiro, Yamada, Toshihiro
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Escherichia, Salmonella Vibrio Staphylococcus aureus Bacillus cereus, The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the and genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of and but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.