X-ray crystal structures of functional ribosome complexes containing transfer RNA and model messenger RNAs and methods of use

X-ray crystal structures of functional ribosome complexes containing transfer RNA and model messenger RNAs and methods of use

5.5 Angstrom resolution x-ray crystallographic structures of 70S ribosome complexes containing messenger RNA and tranfer RNA (tRNA), or tRNA analogs, are provided. The resolution has been enhanced by fitting atomic resolution structures of 30S and 50S subunits onto the 5.5 anstrong electron density...

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Hauptverfasser: Noller, Harry F, Cate, Jamie H. D, Yusupov, Marat M, Yusupova, Gulnara Zh, Baucom, Albion E, Lancaster, Laura, Dallas, Anne, Lieberman, Kathy
Format: Patent
Sprache:eng
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Zusammenfassung:5.5 Angstrom resolution x-ray crystallographic structures of 70S ribosome complexes containing messenger RNA and tranfer RNA (tRNA), or tRNA analogs, are provided. The resolution has been enhanced by fitting atomic resolution structures of 30S and 50S subunits onto the 5.5 anstrong electron density map. The enhanced structure reveals regions of structural differences between the 70S complex and the structures of the individual 30S and 50S components. Pharmacophore design to discover novel inhibitors or activators may be carried out using the enhanced 5.5 Angstrom 70S structure.