Process for producing cis-3-hydroxy-L-proline

Process for producing cis-3-hydroxy-L-proline

An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions t...

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Hauptverfasser: Ozaki, Akio, Mori, Hideo, Shibasaki, Takeshi, Ando, Katsuhiko, Ochiai, Keiko, Chiba, Shigeru, Uosaki, Yoichi
Format: Patent
Sprache:eng
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Zusammenfassung:An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.